Canadian Journal of Plant Science (2008) 88, 633-638

S. Nandy, Q. Chen, J. Yang, D. Beasley, H. Li and M.S. Goettel (2008)
Use of a detached leaf screening method for segregating Colorado potato beetle (Leptinotarsa decemlineata Say) resistant Solanum hybrid lines
Canadian Journal of Plant Science 88 (4), 633-638
Abstract: A diploid segregating population for resistance to Colorado potato beetle (CPB) was developed by crossing the resistant Mexican species Solanum pinnatisectum (S. pnt., 2n = 2x = 24) with the susceptible S. cardiophyllum (S. cph., 2n = 2x = 24). A resistant hybrid plant from this cross was selected and backcrossed as a female to S. cph in order to generate a backcross family. A beetle feeding preference test bioassay was developed to examine segregation for CPB resistance in 345 backcross progeny. A screening method that optimized the length of the feeding period allowed the quantification of CPB resistance in the backcross progeny. Percent resistance of the test lines over the susceptible control was determined using the formula R = 100 x [C/(C + L)] where C and L indicated the amount of damage to the control and test line, respectively, in each Petri dish. All backcross progeny showed an acceptable normal distribution only after 4 and 8 h of feeding. The expression of resistance in the backcross population for this study varied not only among the progeny, but also was influenced by duration of exposure to CPB. A Chi² test of 1R:3S ratio of the 8-h data indicated that CPB resistance was controlled by two genes. Of the five feeding time exposures (4, 8, 12, 20 and 24 h) used in this study, the 8-h exposure was shown to be the optimal time for evaluating resistance to CPB in our potato hybrids.
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Database assignments for author(s): Qin Chen, Mark S. Goettel

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Leptinotarsa decemlineata	Potato (Solanum tuberosum)