Canadian Journal of Microbiology - Revue Canadienne de Microbiologie (1993) 39, 1029-1034

M.S. Mirza, J.L.W. Rademaker, J.D. Janse and A.D.L. Akkermans (1993)
Specific 16S ribosomal RNA targeted oligonucleotide probe against Clavibacter michiganensis subsp. sepedonicus
Canadian Journal of Microbiology - Revue Canadienne de Microbiologie 39 (11), 1029-1034
Abstract: In this article we report on the polymerase chain reaction amplification of a partial 16S rRNA gene from the plant pathogenic bacterium Clavibacter michiganensis subsp. sepedonicus. A partial sequence (about 400 base pairs) of the gene was determined that covered two variable regions important for oligonucleotide probe development. A specific 24mer oligonucleotide probe targeted against the V6 region of 16S rRNA was designed. Specificity of the probe was determined using dot blot hybridization. Under stringent conditions (60 °C), the probe hybridized with all 16 Cl. michiganensis subsp. sepedonicus strains tested. Hybridization did not occur with 32 plant pathogenic and saprophytic bacteria used as controls under the same conditions. Under less stringent conditions (55 °C) the related Clavibacter michiganensis subsp. insidiosus, Clavibacter michiganensis subsp. nebraskensis, and Clavibacter michiganensis subsp. tesselarius also showed hybridization. At even lower stringency (40 °C), all Cl. michiganensis subspecies tested including Clavibacter michiganensis subsp. michiganensis showed hybridization signal, suggesting that under these conditions the probe may be used as a species-specific probe for Cl. michiganensis.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Jan L.W. Rademaker, Jaap D. Janse

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Clavibacter michiganensis
	Clavibacter nebraskensis