Canadian Journal of Botany - Revue Canadienne de Botanique (1991) 69, 1265-1274

Hélène Chamberland, G.B. Ouellette, F.J. Pauzé and P.M. Charest (1991)
Immunocytochemical localization of tomato pectinesterase in root cells of tomato plants infected by Fusarium oxysporum f.sp. radicis-lycopersici
Canadian Journal of Botany - Revue Canadienne de Botanique 69 (6), 1265-1274
Abstract: Polyclonal antibodies produced against a purified commercial pectinesterase obtained from tomato plants permitted ultrastructural localization of this enzyme in the roots of young tomato plants infected by Fusarium oxysporum f.sp. radicis-lycopersici Jarvis and Shoemaker. In inoculated susceptible cultivars, gold labeling for pectinesterase was intense over electrondense material in the cortex intercellular spaces, in altered pit membranes in the vascular cylinder, and in the dense coating material deposited along vessel walls. Contrarily, in resistant or uninoculated susceptible plants, which lacked coating material in vessels, labelling was present but nonabundant in the intercellular spaces. The enzyme was not found in the hyphae, indicating that the labelled pectinesterase was probably of host origin. This study suggests the involvement of the tomato pectinesterase in the host reaction against infection by F. oxysporum f.sp. radicis-lycopersici.
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Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium crown and root rot	Tomato (Solanum lycopersicum)
	Fusarium oxysporum