Bulletin of Insectology (2011) 64, S67-S68

Hana Ludvíková, Jana Fránová and Jana Suchá (2011)
Phytoplasmas in apricot, peach and sour cherry orchards in East Bohemia, Czech Republic
Bulletin of Insectology 64 (Suppl.), S67-S68
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: Symptoms of prematurely yellowing and leaf roll in the summer were observed in apricot, peach and sour cherry orchards in the East Bohemia (Czech Republic) during 2009-2010. Samples from selected symptomatic trees were tested by PCR assays followed by RFLP analyses. Detected phytoplasmas were classified in the apple proliferation group, subgroup 16SrX-B, which representative strains are apricot chlorotic leaf roll and European stone fruit yellows. Although the majority of symptomatic trees was observed in apricot orchards, out of 54 apricot trees examined by PCR/RFLP assays, only 10 plants were positive (18.5%) to phytoplasma presence. In the peach orchards, phytoplasmas were detected in 4 trees out of 14 plants tested (28.6%). Total number of trees examined from sour cherry orchards was 25, out of these 9 plants revealed positive reaction (36%). DNA sequencing of phytoplasma strain from one sour cherry tree confirmed the RFLP identification as `Candidatus Phytoplasma prunorum'. Experiments for transmission of `Ca. P. prunorum' by double budding from positive trees to indicator plants were carried out in open field during 2009 and 2010. No symptoms typical for phytoplasma infection were observed to date.
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Database assignments for author(s): Jana Fránová

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma prunorum	Cherry (Prunus avium - Prunus cerasus)	Czech Republic
	16SrX phytoplasma group	Apricot (Prunus armeniaca)	Czech Republic
	16SrX phytoplasma group	Peach/nectarine (Prunus persica)	Czech Republic