Bulletin of Insectology (2011) 64, S167-S168

Paolo Margaria and Sabrina Palmano (2011)
Proteome responses of Vitis vinifera L. to 'flavescence dorée' phytoplasma infection
Bulletin of Insectology 64 (Suppl.), S167-S168
Second International Phytoplasmologist Working Group Meeting (IPWG), Neustadt an der Weinstraße (Germany) September 12-15, 2011
Abstract: 'Flavescence dorée' is a serious phytoplasma disease affecting grapevine in several European countries. We are currently studying the interaction of 'flavescence dorée' phytoplasma with its natural plant host, by monitoring the effects of infection on the protein expression profile. The red-berried V. vinifera cv. Nebbiolo and Barbera were the source of plant material, as they are widely grown in Piedmont and produce wines of high quality. Moreover, the two varieties show a different sensitivity to the disease: 'Nebbiolo' is generally considered more tolerant to 'flavescence dorée' infection and shows milder symptoms than 'Barbera'. Total proteins from midrib tissues were separated by two dimensional gel electrophoresis, and differentially expressed spots (p ≤ 0.05, ratio > 2) were identified by mass spectrometry (MALDI-TOF-TOF) analysis. The proteins were further analyzed by Blat2GO software, in order to study their function and involvement in biological processes. By this way, we could develop interaction maps, showing the possible involvement of the proteins in several biological pathways.
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Database assignments for author(s): Sabrina Palmano, Paolo Margaria

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	16SrV phytoplasma group	Grapevine (Vitis)