Bulletin of Insectology (2008) 61, 217-218

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Massimo Pajoro, Massimo Marzorati, Ilaria Negri, Luciano Sacchi, Daniele Daffonchio and Alberto Alma (2008)
Investigation over the life cycle of ST1-C the endosymbiont of Scaphoideus titanus
Bulletin of Insectology 61 (1), 217-218
4th European Hemiptera Congress, Ivrea (Turin, Italy) - Sept. 10-14, 2007
Abstract: Scaphoideus titanus Ball (Hemiptera Cicadellidae) is a phloem-feeder leafhopper vector of `Candidatus Phytoplasma vitis', the phytoplasma responsible of 'flavescence dorée' (FD), a grape yellows afflicting several wine production areas in Europe. Natural symbionts of the leafhopper for symbiotic control of FD transmission could be used. Previous work showed the presence of a CFB bacterium, `Candidatus Cardinium sp.' (named ST1-C) in several body districts of S. titanus. Our work was aimed to investigate the life cycle of ST1-C endosymbiont inside and outside of insect body. We fed S. titanus individuals on artificial media and tested insects and diets after the treatment for the presence of the ST1-C endosymbiont by qualitative and quantitative PCR, showing the release of the bacterium during feeding. We also performed fluorescent in situ hybridization on the sugar solutions after insect feeding, detecting ST1-C cells that confirmed its release into the medium. We tested by ST1-C-specific PCR DNA extracted from small areas of grape leaves exposed to S. titanus, identifying the bacterium in the majority of pierced leaves. Our experiments suggest that the ST1-C endosymbiont can undergo a horizontal transmission and have a complex life cycle possibly involving more than one host.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Alberto Alma, Daniele Daffonchio

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.

Scaphoideus titanus