Bulletin of Insectology (2008) 61, 217-218

Massimo Pajoro, Massimo Marzorati, Ilaria Negri, Luciano Sacchi, Daniele Daffonchio and Alberto Alma (2008)
Investigation over the life cycle of ST1-C the endosymbiont of Scaphoideus titanus
Bulletin of Insectology 61 (1), 217-218
4th European Hemiptera Congress, Ivrea (Turin, Italy) - Sept. 10-14, 2007
Abstract: Scaphoideus titanus Ball (Hemiptera Cicadellidae) is a phloem-feeder leafhopper vector of `Candidatus Phytoplasma vitis', the phytoplasma responsible of 'flavescence dorée' (FD), a grape yellows afflicting several wine production areas in Europe. Natural symbionts of the leafhopper for symbiotic control of FD transmission could be used. Previous work showed the presence of a CFB bacterium, `Candidatus Cardinium sp.' (named ST1-C) in several body districts of S. titanus. Our work was aimed to investigate the life cycle of ST1-C endosymbiont inside and outside of insect body. We fed S. titanus individuals on artificial media and tested insects and diets after the treatment for the presence of the ST1-C endosymbiont by qualitative and quantitative PCR, showing the release of the bacterium during feeding. We also performed fluorescent in situ hybridization on the sugar solutions after insect feeding, detecting ST1-C cells that confirmed its release into the medium. We tested by ST1-C-specific PCR DNA extracted from small areas of grape leaves exposed to S. titanus, identifying the bacterium in the majority of pierced leaves. Our experiments suggest that the ST1-C endosymbiont can undergo a horizontal transmission and have a complex life cycle possibly involving more than one host.
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Database assignments for author(s): Alberto Alma, Daniele Daffonchio

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Scaphoideus titanus