Bulletin of Insectology (2007) 60, 255-256

M. Gori, R. Monnanni, M. Buiatti, E. Goti, S. Carnevale, L. Da Prato, A. Bertaccini and S. Biricolti (2007)
Establishing a real-time PCR detection procedure of 'flavescence dorée' and 'bois noir' phytoplasmas for mass screening
Bulletin of Insectology 60 (2), 255-256
First International Phytoplasmologist Working Group Meeting (IPWG), Bologna (Italy) November 12-15, 2007
Abstract: 'Flavescence dorée' (FD) and 'bois noir' (BN) are two grapevine diseases threatening many wine producing countries. Routine detection of FD and BN phytoplasmas mainly rely on nested PCR/RFLP analyses which are time and labour consuming. Realtime PCR can be employed to procedure speed up by coupling specificity of primers and probes and sensitivity of detection system. In this paper a real-time PCR based procedure to detect FD and BN phytoplasmas, suitable for large number of sample testing is described.
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Database assignments for author(s): Assunta Bertaccini

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma vitis	Grapevine (Vitis)	Italy
	Phytoplasma solani	Grapevine (Vitis)	Italy