Bulletin of Insectology (2007) 60, 245-246

M. Seruga Music, M. Krajacic and D. Skoric (2007)
Evaluation of SSCP analysis as a tool for detection of phytoplasma molecular variability
Bulletin of Insectology 60 (2), 245-246
First International Phytoplasmologist Working Group Meeting (IPWG), Bologna (Italy) November 12-15, 2007
Abstract: Single-strand conformation polymorphism (SSCP) analysis is a relatively simple technique broadly used for detecting mutations. The aim of this work was to evaluate the applicability of SSCP analysis as a tool for detection of phytoplasma molecular variability. Seventy phytoplasma isolates from grapevines, belonging to the 16SrXII-A ribosomal subgroup (stolbur) were included in this study. Three different phytoplasma genes were investigated: a part of the 16S rRNA gene, tuf gen and dnaB pseudogene. DNA fragments amplified by PCR were subjected to the SSCP analysis under conditions optimized for each fragment length. SSCP revealed the presence of polymorphisms in the phytoplasma 16S rRNA gene, tuf gene and dnaB pseudogene that was undetectable in routine RFLP analyses. Reliability of the analyses was confirmed by the multiple alignments of representative sequences showing different SSCP profiles, followed by the phylogenetic analyses using various bioinformatics tools.
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Database assignments for author(s): Martina Seruga Music, Dijana Skoric

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma solani	Grapevine (Vitis)	Croatia
	Phytoplasma solani	Grapevine (Vitis)	North Macedonia