Bulletin of Insectology (2007) 60, 115-116

Patricia Carle, Sylvie Malembic-Maher and Xavier Foissac (2007)
Purification of phytoplasma DNA for sequencing the 'flavescence dorée' and stolbur phytoplasma genomes
Bulletin of Insectology 60 (2), 115-116
First International Phytoplasmologist Working Group Meeting (IPWG), Bologna (Italy) November 12-15, 2007
Abstract: In order to compare genomes of the 'flavescence dorée' and stolbur phytoplasmas, we produced fractions from infected plants enriched in phytoplasma DNA. DNA of phytoplasma strains FD92 and stolbur PO were respectively prepared from broad beans and tomato plants by repeated bis-benzymide-CsCl buoyant density gradient centrifugations. Sequence of cloned DNAs were homologous to known phytoplasma sequences in 27 % to 54 % of the cases. According to these data, the sequencing of the genome of these two organisms can be undertaken. A draft of the FD92 phytoplasma genome using the pyrosequencing technology will be first envisaged.
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Database assignments for author(s): Xavier Foissac

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma vitis	Broad/faba bean (Vicia faba)	France
	Phytoplasma solani