Bulletin of Entomological Research (2015) 105, 202-209
Z. Varadínová, Y.J. Wang, Z. Kucerová, V. Stejskal, G. Opit, Y. Cao, F.J. Li and Z.H. Li (2015)
COI barcode based species-specific primers for identification of five species of stored-product pests from genus Cryptolestes (Coleoptera: Laemophloeidae)
Bulletin of Entomological Research 105 (2), 202-209
Abstract: Flat grain beetles of the genus Cryptolestes (Coleoptera: Laemophloeidae) are one of the economically most important stored-product pests which feed on many kinds of agricultural products, especially grains. Nine of more than 40 described Cryptolestes species are recognized as stored-product pests and two of the pest species have a cosmopolitan distribution. Given the rapid growth in global trade of food products, ecological barriers to the spread of pests are easily overcome. Therefore, development of reliable systems for routine quarantine inspection and early infestation detection is vital. In the present study, we established a new rapid and accurate cytochrome c oxidase subunit I-based system for molecular identification of five common stored-product Cryptolestes species, namely, Cryptolestes capensis, Cryptolestes ferrugineus, Cryptolestes pusilloides, Cryptolestes pusillus and Cryptolestes turcicus. Five species-specific primer pairs for traditional uniplex polymerase chain reaction assay are described and their specificity and sensitivity for the identification process is evaluated using larval samples of 12 different populations from three continents (Asia, Europe and North America).
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): George P. Opit, Vaclav Stejskal, Zuzana Kucerova
Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Cryptolestes ferrugineus | ||||
| Cryptolestes pusillus | ||||
| Cryptolestes turcicus | ||||
| Cryptolestes capensis |
