Breeding Science (1994) 44, 1-6

Kikuo Wasano and Shinichi Okuda (1994)
Evaluation of resistance of rice cultivars bacterial grain rot by the syringe inoculation method
Breeding Science 44 (1), 1-6
Abstract: Bacterial grain rot of rice, caused by Pseudomonas glumae, was detected in Fukuoka Prefecture in 1955. Area of infected paddy fields has increased since the 1970's. At present the disease has become one of the most serious rice diseases in the southwestern Japan. But no resistant cultivars have been found. The purpose of the present study was to make varietal differences in resistance to bacterial grain rot clear by establishing new methods of inoculation and evaluation. Seventy five Japanese and 54 foreign rice cultivars in total were used for the experiments in paddy fields of Saga University in 1987 and 1988. Three panicles per plant were inoculated with 0.2 ml of the inoculum per panicle using a syringe at booting stage. The inoculum was adjusted to a concentration of approximately 10⁷cfu/ml . Two weeks after inoculation the disease development was evaluated using two different disease scores, the panicle disease score and spikelet disease score. The correlation coefficient between the two disease scores in 1988 was 0.847. Variances of years, cultivars and interaction be-tween two years were all significant at the 1% level. The correlation coefficient between the spikelet disease scores of 1987 and 1988 was 0.474 which was significant at the 1% level. These results indicate that degree of resistance to Pseudomonas glumae can be evaluated by the new screening methods, even though environmental conditions influence severity of the disease.
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Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Burkholderia glumae	Rice (Oryza)	Japan