Biological Journal of the Linnean Society (2008) 93, 475-486

Flor N. Rivera, Ramón Cisneros, César Hernández-Rodríguez and Gerardo Zúñiga (2008)
Genetic diversity and population structure of Pichia guilliermondii over 400 generations of experimental microevolution
Biological Journal of the Linnean Society 93 (3), 475-486
Abstract: An in vitro evolution model was used to study changes in the genetic diversity of 24 strains of Pichia guilliermondii isolated from the midgut of bark beetles of the genus Dendroctonus. The genetic diversity of P. guilliermondii strains over 400 generations was analysed using multilocus enzyme electrophoresis (MLEE) and random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) markers. Resemblance relationships among strains were observed by cluster analysis. From the MLEE and RAPD markers, it was shown that the effective number of alleles, polymorphism and expected heterozygosity varied over the generations. The average heterozygosity among generations was statistically significant. Both the genetic diversity and the average heterozygosity were statistically significant among generations. The reduction in the population size from 10⁹ to 10⁵ yeast mL⁻¹ associated with each transfer in P. guilliermondii strains and the clonal population structure observed along 400 generations suggest that genetic diversity changes and the observed replacement of genotypes are a consequence of a genetic drift process and not of the reproductive mode.
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Link to article at publishers website
Database assignments for author(s): Gerardo Zuniga

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Dendroctonus (genus)
Meyerozyma guilliermondii (antagonist)