Biocontrol Science and Technology (2016) 26, 227-238

Songqing Wu, Wei Wu, Xiaoli Zhu, Zhaoxia Liu, Carballar-Lejarazú Rebeca, Tao Fu, Chaoqiang Han, Ensi Shao, Xiaohong Pan, Zhipeng Huang, Lei Xu, Shuangquan Zou, Lingling Zhang and Xiong Guan (2016)
Physiological and biochemical response of Aedes aegypti tolerance to Bacillus thuringiensis
Biocontrol Science and Technology 26 (2), 227-238
Abstract: Bacillus thuringiensis subsp. israelensis (Bti) represents the only eco-friendly bio-degradable insecticide for mosquito-borne disease control. Our research aims to identify if mosquito detoxification enzymes play an important role in Bti tolerance mechanisms in the dengue vector, Aedes aegypti. Several enzymes, such as amylase, cytochromes P450, Na⁺/K⁺-ATPase, acetylcholinesterase, protease and glutathione S-transferase (GST) were analysed and level of activity determined in Ae. aegypti larvae after Bti treatment. Bti exposure significantly increased the level of amylase (183.2%) as well as the activity of cytochromes P450 (177.5%), and Na⁺/K⁺-ATPase (142.9%). On the other hand, there was a decrease of 8.6% and 11.4% in acetylcholinesterase and GST activity, and no significant effect in the total level of protease activity. We suggest that the variation in amylase, cytochromes P450, Na⁺/K⁺-ATPase, acetylcholinesterase, protease and GST activity may be associated with the Bti insecticidal mechanism. This study provides the basis of detoxifying enzymes in Bti tolerance.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Xiong Guan

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance to pesticides

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Aedes aegypti
Bacillus thuringiensis (entomopathogen)	Aedes aegypti