Biocontrol Science and Technology (2016) 26, 1-11

Lihua Wei, Miguel Á. Pérez-Rodríguez, Patricia Tamez-Guerra, Erick de Jesús De Luna-Santillana, Ninfa M. Rosas-García, Jesús M. Villegas-Mendoza and Mario A. Rodríguez-Pérez (2016)
Improved insecticidal activity of a genetically modified baculovirus expressing the immunosuppressive CrV1 protein from a polydnavirus against Spodoptera exigua
Biocontrol Science and Technology 26 (1), 1-11
Abstract: Recombinant baculoviruses could be used as biological insecticides through the introduction and expression of exogenous genes (such as those coding for proteins) that interfere with metabolism, metamorphosis (toxins, hormones, and enzymes), and immune system of the insects. The CrV1 secreted protein of Cotesia rubecula polydnavirus (PDV) is responsible for the actin depolymerisation in haemocytes and the abolishment of immune functions such as phagocytosis and cell spreading, thus allowing the successful embryonic development of the parasitoid wasp. CrV1 cDNA was cloned into C6 strain of Autographa californica multiple nucleopolyhedrovirus (AcMNPV-C6-CrV1) under p10 promoter to construct a recombinant virus. The recombinant virus was then tested against the insect pest Spodoptera exigua. The recombinant virus expressing CrV1 protein showed significantly lower LC₅₀ and shorter LT₅₀ as compared with the AcMNPV-C6 wild-type virus. The potential of recombinant baculoviruses expressing PDV genes in relation to their virulence is discussed.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Patricia Tamez-Guerra, Ninfa María Rosas-García, Mario A. Rodríguez-Pérez

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
genetical engin./transgenic plants

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera exigua
Alphabaculovirus aucalifonicae (entomopathogen)	Spodoptera exigua