Archives of Virology (2017) 162, 505-510

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Wenwu Lin, Wenkai Yan, Wenting Yang, Chaowei Yu, Huihuang Chen, Wen Zhang, Zujian Wu, Liang Yang and Lianhui Xie (2017)
Characterisation of siRNAs derived from new isolates of Bamboo mosaic virus and their associated satellites in infected ma bamboo (Dendrocalamus latiflorus)
Archives of Virology 162 (2), 505-510
Abstract: We characterised the virus-derived small interfering RNAs (vsiRNA) of bamboo mosaic virus (Ba-vsiRNAs) and its associated satellite RNA (satRNA)-derived siRNAs (satsiRNAs) in a bamboo plant (Dendrocalamus latiflorus) by deep sequencing. Ba-vsiRNAs and satsiRNAs of 21–22 nt in length, with both (+) and (-) polarity, predominated. The 5'-terminal base of Ba-vsiRNA was biased towards A, whereas a bias towards C/U was observed in sense satsiRNAs, and towards A in antisense satsiRNAs. A large set of bamboo genes were identified as potential targets of Ba-vsiRNAs and satsiRNAs, revealing RNA silencing-based virus-host interactions in plants. Moreover, we isolated and characterised new isolates of bamboo mosaic virus (BaMV; 6,350 nt) and BaMV-associated satRNA (satBaMV; 834 nt), designated BaMV-MAZSL1 and satBaMV-MAZSL1, respectively.
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Database assignments for author(s): Zu-Jian Wu

Research topic(s) for pests/diseases/weeds:
molecular biology - genes


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Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Bamboo mosaic virus Bamboo