Archives of Virology (2017) 162, 2791-2796

Reetika Kapoor, Nishant Srivastava, Shailender Kumar, R.K. Saritha, Susheel Kumar Sharma, Rakesh Kumar Jain and Virendra Kumar Baranwal (2017)
Development of a recombinase polymerase amplification assay for the diagnosis of Banana bunchy top virus in different banana cultivars
Archives of Virology 162 (9), 2791-2796
Abstract: Recombinase polymerase amplification (RPA) is a rapid, isothermal amplification method with high specificity and sensitivity. In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both in RPA as well as in polymerase chain reaction (PCR). A total of 133 symptomatic as well as asymptomatic banana leaf samples from various cultivars were collected from the different regions of India and evaluated for BBTV infection using the RPA assay. BBTV was efficiently detected using crude leaf sap in RPA and the results obtained were consistent with PCR-based detection using purified DNA as template. To our knowledge, this is the first report of reliable diagnosis of BBTV infection by RPA using crude leaf sap as a template.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Virendra Kumar Baranwal, Rakesh K. Jain

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Banana bunchy top virus	Banana/plantain (Musa)	India