Archives of Virology (2013) 158, 1181-1187

Beibei Ge, Qian Li, Guojie Liu, Meiguang Lu, Shifang Li and Hongqing Wang (2013)
Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR
Archives of Virology 158 (6), 1181-1187
Abstract: Potato virus M (PVM), pepino mosaic virus (PepMV), tomato mosaic virus (ToMV), and potato virus S (PVS) infect pepino and cause serious crop losses. In this study, a multiplex RT-PCR method was developed for simultaneous detection and differentiation of PVS, ToMV, PepMV and PVM. The method was highly reliable and sensitive; validation was accomplished by testing pepino samples collected from different regions of China. In this survey, PVM, ToMV and PVS were detected in 37.0 %, 31.0 % and 5.5 % of samples tested, respectively, confirming the widespread occurrence of these three viruses in China. PepMV was not detected in any of the samples, which indicated that this virus may not be prevalent in China. The results suggest that the new multiplex RT-PCR method has potential to be used routinely for surveys of pepino for virus infection.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Shi-Fang Li

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tomato mosaic virus	Solanum (crop)
	Potato virus S	Solanum (crop)
	Potato virus M	Solanum (crop)
	Pepino mosaic virus	Solanum (crop)