Archives of Virology (2001) 146, 1841-1853

J. Chen, J. Chen and M.J. Adams (2001)
Molecular characterisation of a complex mixture of viruses in garlic with mosaic symptoms in China
Archives of Virology 146 (10), 1841-1853
Abstract: Degenerate primers were used to detect and amplify cDNA of viruses of the genera Carlavirus, Allexivirus and Potyvirus from garlic plants with mosaic symptoms growing in Zhejiang province, China. Plants contained a complex mixture of viruses and strains. Three distinct stains of Garlic latent virus were detected; the most frequent one was completely sequenced and partial sequences were obtained for the other two. The complete sequence (8363 nt) was 76.4% identical to a Korean isolate. Two allexiviruses were detected and completely sequenced. One (8319 nt) was identified as Garlic virus X and comparisons showed that a published Korean isolate (which had 90.2% identical nucleotides) had an N-terminal deletion in the serine-rich ORF4. The other isolate (8451 nt), tentatively named Garlic virus E, appeared to be a new member of the genus. Phylogenetic analyses of the different viral proteins and distinctive conserved sequence motifs within the genus are discussed. This is the first report of allexiviruses from China. Using potyvirus primers, three distinct isolates of Onion yellow dwarf virus and one of Leek yellow stripe virus were detected and the 3'-terminal sequences of their genomes were determined. In a coat protein phylogenetic analysis, the new isolates were most closely related to other published isolates from Japan and China.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jiong Chen, Jianping Chen

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Onion yellow dwarf virus	Onion/garlic/leek (Allium)	China (south)
	Leek yellow stripe virus	Onion/garlic/leek (Allium)	China (south)
	Garlic common latent virus	Onion/garlic/leek (Allium)	China (south)
	Garlic virus E	Onion/garlic/leek (Allium)	China (south)