Archives of Virology (1996) 141, 185-195

S.N. Salm, M.E.C. Rey and R. French (1996)
Molecular cloning and nucleotide sequencing of the 3'-terminal region of a South African isolate of Ryegrass mosaic virus RNA and in vitro expression of the coat protein gene
Archives of Virology 141 (1), 185-195
Abstract: The 2094 nucleotides at the 3'-terminus of a South African isolate of ryegrass mosaic virus (RGMV) was cloned and sequenced. Two putative poly-protein cleavage sites were found: Q/L and E/A, both of which are novel in the Potyviridae. The RGMV-SA cDNA was cloned into an expression vector, pUEX, and a fusion protein of 185 kDa was obtained which reacted strongly to anti-RGMV-SA antiserum. Alignment of the predicted amino acid sequence of RGMV-SA with those of other Potyviridae members showed limited identity, indicating that RGMV-SA is a definite and distinct virus.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Roy French, Marie Emma Christine Rey

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ryegrass mosaic virus		South Africa