Archives of Phytopathology and Plant Protection (2019) 52, 917-929

Marzieh Shamshiri, Jahangir Heydarnejad, Mehdi Kamali, Najmeh Pouramini and Hossain Massumi (2019)
Identification of wild hosts of Tomato yellow leaf curl virus in South-Eastern Iran
Archives of Phytopathology and Plant Protection 52 (9-10), 917-929
Abstract: In this research, to identify natural wild hosts of TYLCV, 44 symptomatic samples were collected in or around harvested tomato fields in south-eastern Iran and tested for the TYLCV infection by PCR. Among four PCR-positive plant species, full-length genome of TYLCV was amplified by rolling circle amplification (RCA) method only from a ground cherry (Physalis divaricata L., Solanaceae) sample. Cloning and full-length genome sequencing of a ground cherry isolate (G4) showed that it comprises 2756 nucleotides (nts) in length and shares 87.7%–95.2% nt sequence identities with TYLCV isolates reported from Iran, Oman and Pakistan. The G4 isolate of TYLCV was successfully transmitted to tomato plants and the original host (P. divaricata) via agroinoculation and showed typical symptoms. According to the results of this research, four symptomatic weed species appear to be alternative hosts of TYLCV and play the role of the primary inoculum for the viral infection.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jahangir Heydarnejad, Hossain Massumi

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tomato yellow leaf curl virus	Tomato (Solanum lycopersicum)	Iran