Applied and Environmental Microbiology (2018) 84 (17 - e01089-18)
Isabel Gómez, Daniel E. Rodríguez-Chamorro, Gabriela Flores-Ramírez, Ricardo Grande, Fernando Zúñiga, Francisco J. Portugal, Jorge Sánchez, Sabino Pacheco, Alejandra Bravo and Mario Soberón (2018)
Spodoptera frugiperda (J. E. Smith) aminopeptidase N1 is a functional receptor of the Bacillus thuringiensis Cry1Ca toxin
Applied and Environmental Microbiology 84 (17 - e01089-18)
Abstract: Bacillus thuringiensis Cry1Ca is toxic to different Spodoptera species. The aims of this work were to identify the Cry1Ca-binding proteins in S. frugiperda, to provide evidence on their participation in toxicity, and to identify the Cry1Ca amino acid residues involved in receptor binding. Pulldown assays using Spodoptera frugiperda brush border membrane vesicles (BBMV) identified aminopeptidase N (APN), APN1, and APN2 isoforms as Cry1Ca-binding proteins. Cry1Ca alanine substitutions in all residues of domain III β16 were characterized. Two β16 nontoxic mutants (V505A and S506A) showed a correlative defect on binding to the recombinant S. frugiperda APN1 (SfAPN1). Finally, silencing the expression of APN1 transcript, by double-stranded RNA (dsRNA) feeding, showed that silenced larvae are more tolerant of the Cry1Ca toxin, which induced less than 40% mortality in silenced larvae whereas nonsilenced larvae had 100% mortality. Overall, our results show that Cry1Ca relies on APN1 binding through domain III β16 to impart toxicity to S. frugiperda.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Alejandra Bravo
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Spodoptera frugiperda | ||||
| Bacillus thuringiensis Cry1C-toxin (entomopathogen) | Spodoptera frugiperda |
