Applied and Environmental Microbiology (2009) 75, 2945-2950

Jennifer Hodgetts, Neil Boonham, Rick Mumford and Matthew Dickinson (2009)
Panel of 23S rRNA gene-based real-time PCR assays for improved universal and group-specific detection of phytoplasmas
Applied and Environmental Microbiology 75 (9), 2945-2950
Abstract: Primers and probes based on the 23S rRNA gene have been utilized to design a range of real-time PCR assays for routine phytoplasma diagnostics. These assays have been authenticated as phytoplasma specific and shown to be at least as sensitive as nested PCR. A universal assay to detect all phytoplasmas has been developed, along with a multiplex assay to discriminate 16SrI group phytoplasmas from members of all of the other 16Sr groups. Assays for the 16SrII, 16SrIV, and 16SrXII groups have also been developed to confirm that the 23S rRNA gene can be used to design group-specific assays.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jennifer Hodgetts, Neil Boonham

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	16SrI phytoplasma group
	16SrII phytoplasma group
	16SrXII phytoplasma group
	16SrIV phytoplasma group