Applied and Environmental Microbiology (2006) 72, 7029-7035

R. Monnerat, E. Martins, P. Queiroz, S. Ordúz, G. Jaramillo, G. Benintende, J. Cozzi, M.D. Real, A. Martinez-Ramirez, C. Rausell, J. Cerón, J.E. Ibarra, M.C. Del Rincon-Castro, A.M. Espinoza, L. Meza-Basso, L. Cabrera, J. Sánchez, M. Soberon and A. Bravo (2006)
Genetic variability of Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) populations from Latin America is associated with variations in susceptibility to Bacillus thuringiensis Cry Toxins
Applied and Environmental Microbiology 72 (11), 7029-7035
Abstract: Bacillus thuringiensis strains isolated from Latin American soil samples that showed toxicity against three Spodoptera frugiperda populations from different geographical areas (Mexico, Colombia, and Brazil) were characterized on the basis of their insecticidal activity, crystal morphology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of parasporal crystals, plasmid profiles, and cry gene content. We found that the different S. frugiperda populations display different susceptibilities to the selected B. thuringiensis strains and also to pure preparations of Cry1B, Cry1C, and Cry1D toxins. Binding assays performed with pure toxin demonstrated that the differences in the toxin binding capacities of these insect populations correlated with the observed differences in susceptibility to the three Cry toxins analyzed. Finally, the genetic variability of the three insect populations was analyzed by random amplification of polymorphic DNA-PCR, which showed significant genetic diversity among the three S. frugiperda populations analyzed. The data presented here show that the genetic variability of S. frugiperda populations should be carefully considered in the development of insect pest control strategies, including the deployment of genetically modified maize in different geographical regions.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Alejandra Bravo, Jorge E. Ibarra, Sergio Orduz, Maria Dolores Real, Jairo Cerón, Luís Meza-Basso

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Spodoptera frugiperda
Bacillus thuringiensis Cry1D-toxin (entomopathogen)
Bacillus thuringiensis Cry1B-toxin (entomopathogen)
Bacillus thuringiensis Cry1C-toxin (entomopathogen)