Applied and Environmental Microbiology (1994) 60, 4094-4099

S. Manulis, L. Valinsky, A. Lichter and D.W. Gabriel (1994)
Sensitive and specific detection of Xanthomonas campestris pv. pelargonii with DNA primers and probes identified by random amplified polymorphic DNA analysis
Applied and Environmental Microbiology 60 (11), 4094-4099
Abstract: The random amplified polymorphic DNA method was used to distinguish strains of Xanthomonas campestris pv. pelargonii from 21 other Xanthomonas species and/or pathovars. Among the 42 arbitrarily chosen primers evaluated, 3 were found to reveal diagnostic polymorphisms when purified DNAs from compared strains were amplified by the PCR. The three primers revealed DNA amplification patterns which were conserved among all 53 strains tested of X. campestris pv. pelargonii isolated from various locations worldwide. The distinctive X. compestris pv. pelargonii patterns were clearly different from those obtained with any of 46 other Xanthomonas strains tested. An amplified 1.2-kb DNA fragment, apparently unique to X. campestris pv. pelargonii by these random amplified polymorphic DNA tests, was cloned and evaluated as a diagnostic DNA probe. It hybridized with total DNA from all 53 X. campestris pv. pelargonii strains tested and not with any of the 46 other Xanthomonas strains tested. The DNA sequence of the terminal ends of this 1.2-kb fragment was obtained and used to design a pair of 18-mer oligonucleotide primers specific for X. campestris pv. pelargonii. The custom-synthesized primers amplified the same 1.2-kb DNA fragment from all 53 X. campestris pv. pelargonii strains tested and failed to amplify DNA from any of the 46 other Xanthomonas strains tested. DNA isolated from saprophytes associated with the geranium plant also did not produce amplified DNA with these primers. The sensitivity of the PCR assay using the custom-synthesized primers was between 10 and 50 cells. The techniques used could provide a general method to identify pathovar-specific DNA primers for rapid, sensitive, and specific detection and identification of plant-pathogenic bacteria.
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Database assignments for author(s): Dean W. Gabriel, Amnon Lichter

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas hortorum pv. pelargonii		Germany
	Xanthomonas hortorum pv. pelargonii		Israel
	Xanthomonas hortorum pv. pelargonii		Mexico
	Xanthomonas hortorum pv. pelargonii		U.S.A. (NE)
	Xanthomonas hortorum pv. pelargonii		U.S.A. (SE)