Applied Entomology and Zoology (2012) 47, 95-101

Qiang Fu, Yukiko Matsumoto, Masaya Matsumura, Yoshio Hirai, Yuki Sato and Hiroaki Noda (2012)
Presence of a short repeat sequence in internal transcribed spacer (ITS) 1 of the rRNA gene of Sogatella furcifera (Hemiptera: Delphacidae) from geographically different populations in Asia
Applied Entomology and Zoology 47 (2), 95-101
Abstract: Nucleotide sequences of the internal transcribed spacer 1 (ITS1)-5.8S-ITS2 region of the nuclear ribosomal RNA gene were determined in the white-backed planthopper (WBPH) Sogatella furcifera (Horváth) to detect molecular variation among regional populations in Asia. We analyzed 932 sequences from 172 individuals (4-9 clones per individual) of 33 populations collected in 1987-2008 from six countries, Japan, China, Taiwan, Vietnam, Philippines, and Papua New Guinea. WBPH showed intra-individual variation in ITS1, which is mainly attributable to the frequency (0-10) of the 66-bp repeat sequence in ITS1. Among the examined clones, the sequences of 5.8S were mostly identical and those of ITS2 were similar. A single planthopper had a maximum of 6 different variants in the number of ITS1 repeats, suggesting highly varied repeat numbers in individual planthoppers. The ITS1 with four repeats was the most frequently (64%) detected. Such a repeat was not observed in two other economically important planthopper species, Nilaparvata lugens (Stål) and Laodelphax striatellus (Fallén). The ITS nucleotide sequences in the WBPH populations in Asia were genetically close and some variations in the sequences were not related to regional populations, indicating that the nucleotide sequences of the ITS region are not useful for geographical discrimination of the WBPH. This closeness seems to be caused by long distance migration and genetic exchange among populations.
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Link to article at publishers website
Database assignments for author(s): Qiang Fu, Masaya Matsumura

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sogatella furcifera		Japan
	Sogatella furcifera		Papua New Guinea
	Sogatella furcifera		Philippines
	Sogatella furcifera		Taiwan
	Sogatella furcifera		Vietnam