Applied Entomology and Zoology (1999) 34, 497-500

Jun Ohnishi, Daijirou Hosokawa, Tamotsu Murai and Shinya Tsuda (1999)
A simple rearing system for Thrips setosus Moulton (Thysanoptera: Thripidae) using a leaf cage method for the transmission experiment of tomato spotted wilt tospovirus
Applied Entomology and Zoology 34 (4), 497-500
Abstract: We have developed a simple rearing system for Thrips setosus to facilitate the collection and maintenance of a large number of even-aged for use in transmission experiments of tomato spotted wilt tospovirus. The system was established using a leaf cage. The leaf cage consisted of an excised leaf of the plant, Vigna susquipedaris, sandwiched between two acrylic plastic plates held by rubber bands. The upper plate; had a 25 mm-diameter hole in the center and two air ventilation holes, while the lower plate was flat. In this cage, approximately 75% of the total egg number could be collected during the first 6 h of a 24 h oviposition period, indicating that the first 6 h of the oviposition period is practical to collect eggs efficiently using this method. On day 6 after egg oviposition, 54.4% of the total number of hatched larvae was observed in the former half of the photophase (within 8 h after onset of the photophase), suggesting that this period is efficient for collection of larvae with synchronized age. This method was useful not only to collect a lot of synchronously hatched first instar larvae, but also to maintain them until adulthood without escape from the cage.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Tamotsu Murai, Jun Ohnishi

Research topic(s) for pests/diseases/weeds:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Thrips setosus		Japan