Annals of the Entomological Society of America (1999) 92, 56-62
Nuria Agustí, José Aramburu and Rosa Gabarra (1999)
Immunological detection of Helicoverpa armigera (Lepidoptera : Noctuidae) ingested by heteropteran predators: Time-related decay and effect of meal size on detection period
Annals of the Entomological Society of America 92 (1), 56-62
Abstract: Indirect enzyme-linked immunosorbent assay (ELISA), dot blot, and squash blot were compared for sensitivity and ease of use for detecting Helicoverpa armigera (Hübner) egg antigen using an egg-specific monoclonal antibody. The best results were obtained by dot blot, although several indirect ELISA conditions were tried. Three of the main predator species in Mediterranean vegetable crops--Dicyphus tamaninii Wagner and Macrolophus caliginosus Wagner (Hemiptera: Miridae), and Orius majusculus (Reuter) (Hemiptera: Anthocoridae)--which had fed on H. armigera eggs, were assayed by dot blot to detect egg antigen in their gut. The prey was detected in all samples. Another study was conducted to determine the effect of meal size and time after feeding on the prey detection period in D. tamaninii. The proportion of prey remaining at various time intervals after feeding was measured. Curves were fitted to exponential and linear models and detection periods were estimated. The decline in prey detection over time was fitted with an exponential model for predators that ate 1 egg and a linear model for predators that ate 10 eggs. Therefore, meal size produces a considerable difference in the rate of decline and the estimated detection periods.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Nuria Agusti, Rosa Gabarra
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
surveys/distribution/isolation
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Helicoverpa armigera | ||||
| Dicyphus tamaninii (predator) | Helicoverpa armigera | |||
| Macrolophus melanotoma (predator) | Helicoverpa armigera | |||
| Orius majusculus (predator) | Helicoverpa armigera |
