Annals of Applied Biology (2020) 177, 374-384

Elinor Darzi, Oded Lachman, Elisheva Smith, Amnon Koren, Eyal Klein, Nadav Pass, Omer Frenkel and Aviv Dombrovsky (2020)
Paths of Cucumber green mottle mosaic virus disease spread and disinfectant-based management
Annals of Applied Biology 177 (3), 374-384
Abstract: Cucumber green mottle mosaic virus (CGMMV) assigned to the genus Tobamovirus is considered a major disease cause of cucurbits worldwide. A primary route for CGMMV disease spread is via mechanical contact. The virus is highly stable and adheres to various agricultural equipment. In the current study, we examined means to inactivate the virus and reduce disease spread via planting equipment and supplies using various chemicals. We have found that incubations of CGMMV-infected cucumber plant extracts with MENNO-Florades 2%, Virocid 3% or Green Up D 20% inactivated the virus and prevented disease spread in a biological assay. Stabilised chlorine formulation (KlorBac), which has the active ingredient troclosene-sodium (sodium dichloroisocyanurate, SDIC) at 2,000 ppm, was efficient in disinfecting CGMMV-contaminated grafting knives in 2 s. Similarly, immersing virus contaminated grafting knives for 2 s in 20% (wt/vol) non-fat milk powder reduced infectivity of the contaminated knives. CGMMV-contaminated nursery sowing trays could constitute a primary infectious viral source transmitted via irrigation water. CGMMV-contaminated sowing trays immersed in KlorBac 2,000 ppm or active oxygen (Huwa-San TR-50) 1%, were efficiently disinfected. Interestingly, hydrophobic insulation of the CGMMV-contaminated trays using dry silicone layers reduced initiation of the viral primary infection in CGMMV-contaminated new sowing trays but was less efficient in CGMMV-contaminated re-used trays. Importantly, Septadine (0.5% chlorhexidine gluconate) was not effective in disinfection of grafting knives. Notably, CGMMV-infected cucumber plant extract incubated with 20% (wt/vol) non-fat milk powder was refractory to the milk suggesting that virus release from surfaces did not necessarily involve virus inactivation.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Aviv Dombrovsky

Research topic(s) for pests/diseases/weeds:
control - general
transmission/dispersal of plant diseases

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber green mottle mosaic virus		Israel