Agronomy Research (2006) 4, 151-154

D. Burokiene (2006)
Early detection of Clavibacter rnichiganensis subsp. michiganensi in tomato seedlings
Agronomy Research 4 (special issue), 151-154
Abstract: The investigations on Clavibacter michiganensis subsp. michiganensis in tomato seedlings were carried out using the BIO-PCR method. One to two (1-2) colonies were already detectable with species-specific primers CMM5 and CMM6 using Clavibacter michiganensis subsp. michiganensis bacteria in plant seedlings. The method allowed detection of bacteria at a distance of 8 cm farthest from the inoculation site in 95% of samples 3 days after treatment, at the earliest. Plant seedlings approximately 40 cm high were infected at full length after 9 days. Experimental results indicated the possibility of detecting the pathogen in very early stages of infection. Therefore, the BIO-PCR method was a highly specific, rapid and reliable detection technique, which might help to control the spread of the pathogen to healthy tomato plants.
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Database assignments for author(s): Daiva Burokiene

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Clavibacter michiganensis	Tomato (Solanum lycopersicum)	Lithuania