Acta Entomologica Sinica (2008) 51, 714-719
Fang Tang, Hai-Jing Yang, Xi-Wu Gao and Ao-Jin Yan (2008)
[Establishment of optimization of assaying activity of acetylcholinesterase from Odontotermes formosanus (Isoptera : Termitidae) and their sensitivity to insecticides]
Acta Entomologica Sinica 51 (7), 714-719
Abstract: The optimal conditions for assaying the activity of acetylcholinesterase (AChE) from Odontotermes formosanus (Shiraki) were determined by orthogonal experiments. The concentrations of acetylcholinesterase and substrate, pH and temperature of the reaction system) and reaction time were optimized. L25 (56) orthogonal matrix was adopted without considering interaction. Through analyzing the data from orthogonal experiment with range analysis and variance analysis, we found that the optimal conditions were as enzyme concentration 12.5 g/L, substrate concentration 8 mmol/L, pH 8.0, reaction temperature 40°C , and reaction time 5 min. Furthermore, the inhibition of 6 insecticides to AChE from O. formosanus was determined. The IC50 values of methomyl, phoxim, triazophos, profenofos, malathion and omethoate were 3.52 x 10-4, 1.86 x 10-3, 5.13 x 10-3, 9.55 x 10-4 , 8.81 x 10-3 and 1.39 x 10-2 mol/L, respectively. The inhibition of ACNE activity by six insecticides increased with the increasing of insecticide concentration from 3.3 x 10-7 to 5 x 10-3 mol/L.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Xi-Wu Gao
Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
control - general
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Odontotermes formosanus |
