Acta Entomologica Sinica (2008) 51, 1235-1243

Ming Li, Wen-Cai Lu, Hong-Zu Feng, Liang Yuan, Jin-Jun Wang and Lin He (2008)
[Cloning and expression analysis of a heat shock protein 70 gene TCHSP70-4 from Tetranychus cinnabarinus (Acari : Tetranychidae)]
Acta Entomologica Sinica 51 (12), 1235-1243
Abstract: In order to study the correlation between the expression of heat shock protein 70 (HSP70) and the adaptation to high and low temperature stress of Tetranychus cinnabarinus (Boisduval), the cDNA of a HSP70 gene named TCHSP70-4 was cloned by using the species homology and technique of rapid amplification of cDNA end (RACE) from carmine spider mite. The full-length of TCHSP70-4 cDNA (GenBank accession no. : EU977182) was 2 182 by and the open reading frame ( ORF) was 1 959 by encoding a polypeptide of 653 amino acids, with an estimated molecular mass of 70.9 kDa and an estimated isoelectric piont ( pl) of 5.40. It contained the highly conserved functional motifs of the HSP70 family. Comparative quantitative real-time RT-PCR was used to analyze the expression of TCHSP70-4 gene after the treatments of cold shock (4 °C ) and heat shock (40°C ) for 1 h, respectively. The results showed that the mRNA expression of TCHSP70-4 was significantly down-regulated after cold shock and significantly up-regulated after heat shock. The result of TCHSP70-4 gene expression, on one hand, revealed that the gene is of induced HSP70 gene; on the other hand, it also revealed that its expression and protective effect were different after cold stress and heat stress.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Lin He, Jin-Jun Wang, Hongzu Feng

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tetranychus cinnabarinus