Acta Entomologica Sinica (2008) 51, 120-125

Ying-Jun Zhou, Yi-Hua Yang, Shu-Wen Wu and Yi-Dong Wu (2008)
[Cloning and sequence analysis of the 5'-upstream region of cytochrome P450 gene CYP9A 12 of Helicoverpa armigera (Hübner)]
Acta Entomologica Sinica 51 (2), 120-125
Abstract: Overexpression of cytochrome P450 CYP9A 12 gene has been proved to be associated with pyrethroid resistance in the cotton bollworm, Helicoverpa armigera. Based on the 5'-end nucleotide sequence of the full length cDNA of CYP9A 12, the 5'-upstream region of CYP9A 12 was obtained by genome walking technique to investigate the regulatory mechanism of CYP9A12 gene expression. The 5'-upstream fragment of 3 575 by was successfully cloned and sequenced and there is an intron (2 124 bp) located 3 by upstream of the start codon. The transcription initiation site of CYP9A 12 predicted by the NNPP software is consistent with that deduced from the full length cDNA of CYP9A 12. TFSEARCH 1.3 software predicted several cis-acting element binding sites in the 5'-upstream region of CYP9A12, including not only the core promoter sequences (TATA-box and CAAT-box , but also several transcriptional elements (GATA-1, CdxA, Dfd, etc.). The current study will provide an important basis for further investigating regulation of CYP9A 12 expression and molecular mechanism of CYP9A 12 involved in insecticide resistance of H. armigera in future.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Yihua Yang

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera