Acta Entomologica Sinica (2008) 51, 1138-1143

Hong-Bo Li and Yu-Xian Xia (2008)
[Expression and insecticidal activity of insect-specific neurotoxin Bj-alpha-IT]
Acta Entomologica Sinica 51 (11), 1138-1143
Abstract: According to the codon bias of Pichia pastoris. the insect-specific neurotoxin gene Bj-alpha-IT was synthesized based on its amino acid sequence and was cloned to vectors of PET-30a ( + ) and pPIC9K respectively. The fusion protein of Bj-alpha-IT expressed in Escherichia coli was induced with IPTG and was purified with Ni-NTA His Bind Column. The purified fusion protein was used to prepare antiserum and conduct bioactivity test. Dot blotting was used to screen the high-level expressed transformants of P. pastoris. The results showed that the highest expression of recombinant Bj-alpha-IT in P. pastoris was about 20 mg/L in baffled flasks, and the Bj-alpha-IT fusion protein expressed in E. coli was not toxic to locust Locusta migratoria manilensis and cockroach Blattella germanica, but that expressed in P. pastoris had insecticidal activity against locust and cockroach through injection.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Yuxian Xia

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Blattella germanica
	Locusta migratoria