Acta Entomologica Sinica (2007) 50, 788-794

Zhao-Nong Hu, Bing-Jun He, Yu-Zhe Du, An-Xi Liu and Wen-Jun Wu (2007)
[Effects of celangulin IV and V on sodium channels in nerve cells of cotton bollworm (Helicoverpa armigera)]
Acta Entomologica Sinica 50 (8), 788-794
Abstract: As the key structure of excitability in biological systems, the voltage-gated sodium channel is targeted by a series of neurotoxins derived from plants. Celangulin IV and V isolated from the root bark of Celastrus angulatus are the major active ingredients of the insecticidal plant which can result in reverse nerve poisoning symptoms, i.e., narcosis and excitation, respectively. Their effects on inward sodium channel current in nerve cells of cotton bollworm Helicoverpa armigera were investigated in vitro by using patch clamp technique. The results indicated that celangulin IV reduced tetrodotoxin (TTX)-sensitive inward sodium current in a concentration dependent manner. The sodium peak current was reduced immediately in high concentration (10 and 1 µmol/L), and decreased slowly in middle concentration (0.1 µmol/L). In low concentration(0.01 µmol/L), however, the peak current was increased in the beginning, and then reduced. Moreover, it had no effect on activated voltage, but the peak voltage shifted to positive potential which was dependent on the concentration of celangulin IV. After treatment with celangulin V, the TTX-sensitive inward sodium channel current was increased distinctly in a concentration dependant manner. It had no effect on activated voltage, but the peak voltage shifted to positive potential in lower concentration (0.1 and 0.01 µmol/L) of celangulin V. Due to their chemical structure difference, celangulin IV and V could result in qualitatively divergent kinetics of sodium channels at a shared binding site, so their physiological effects are different.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Bing-Jun He

Research topic(s) for pests/diseases/weeds:
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera