Acta Entomologica Sinica (2006) 49, 562-567

Yan-Ling Xu, Zhen-Ying Wang, Kang-Lai He and Shu-Xiong Bai (2006)
[Effects of transgenic Bt corn expressing Cry1Ab toxin on activities of some enzy mes in larvae of the Asian corn borer, Ostrinia furnacalis (Guenée) (Lepidoptera: Pyralidae)]
Acta Entomologica Sinica 49 (4), 562-567
Abstract: The activities of three detoxification enzymes (alpha-naphthyl acetate esterase, acetylcholinesterase and glutathione-S-transferase), two protective enzymes (superoxide dismutase and catalase) and midgut proteases (total protease, trypsin-like enzyme and chymotrypsin-like enzyme) in 3rd instar larvae of the Asian corn borer, Ostrinia furnacalis after feeding on Bt-transgenic corn were evaluated in the laboratory. The results showed that the activities of alpha-naphthylacetate esterase, glutathione-S-transferase in the larvae fed on Bt corn leaf for 48 h were greatly lower than that of the control fed on the non-transgenic corn. However, the acetylcholinesterase activity of the larvae fed on Bt corn for 48 h, 60 h and 72 h increased markedly, being 2.00-, 1.50- and 2.50-fold higher than that of the control, respectively. The activities of protective enzymes, total protease, weak trypsin-like enzyme and chymotrypsin-like enzyme in the larvae fed on Bt corn for 48 h were also significantly lower than that of the control. However, the activities of the active alkaline trypsin-like enzyme were obviously higher than those of the control. The active alkaline trypsin-like enzyme activities of the larvae fed on Bt corn for 48 h, 60 h and 72 h were 4.00-, 1.67- and 1.33-fold higher than that of the control. The results suggest that acetylcholinesterase and active alkaline trypsin-like enzyme may be associated with Asian corn borer resistance to Bt.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zhen-Ying Wang, Kanglai He

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ostrinia furnacalis	Maize/corn (Zea mays)