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Acta Entomologica Sinica (2005) 48, 871-875
Qin Yao, Lu Gao, Ke-Ping Chen and Zhi-Gang Hu (2005)
[Detection of proliferation of Bombyx mori nucleopolyhedrovirus in its host by fluorescence quantitative PCR]
Acta Entomologica Sinica 48 (6), 871-875
Abstract: To investigate the proliferation of Bombyx mori nucleopolyhedrovirus (BmNPV) in its host, the 5th instar larvae of the susceptible silkworm strain 306 were orally administered with determinate dose of BmNPV. After oral ingestion, midgut, haemolymph and fat body tissues were collected at different time to extract DNA. Fluorescence quantitative PCR amplification of BmNPV DNA polymerase gene (dnapol) was used to detect copy number of BmNPV, and each sample was normalized using silkworm cytoplasm actin A3. The results showed that BmNPV could be detected in the midgut 2 hours post infection. BmNPV could be detected in the haemolymph and fat body 12 hours post infection. Through a latent period of 12 hours, rapid proliferation of virus was detected in the midgut, haemolymph and fat body. The proliferation of BmNPV entered a stationary phase 84 hours post infection.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Alphabaculovirus bomori |