Acta Entomologica Sinica (2005) 48, 498-502

Xiao-Hong Huang, Qing-Xi Chen, Min-Sheng You, Jun Wang and Xiong Guan (2005)
[Isolation, purification and partial characterization of the ß-N-acetyl-D-glucosaminidase from the pupae of Helicoverpa armigera]
Acta Entomologica Sinica 48 (4), 498-502
Abstract: ß-N-acetyl-D-glucosaminidase (EC3.2.1.52) was purified from the pupae of Helicoverpa armigera by ammonium sulfate fractionation and chromatography on Sephadex G-200 and DEAE cellulose. The purified enzyme preparation was homogeneous as judged by polyacrylamide gel electrophoresis. It was found that the specific activity of the enzyme was 2 678.79 U/mg. The optimal pH value was 5.63 and the optimal temperature 55°C. The enzyme was stable in the pH ranges of 4 to 8 under 37°C. The enzyme follows typical Michaelis Menten kinetics for the hydrolysis of pNP-ß-D-GlcNAc and the Km and Vm values were 0.16 mmol/L and 10.73 µmol.L^-1·min^-1, respectively. The activation energy of the enzyme for the hydrolysis of pNP-ß-D-GlcNAc was 66.24 kJ/mol.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Xiong Guan, Minsheng You

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera