Acta Entomologica Sinica (2005) 48, 353-358

Qin-Ying Wang, Zi-Yan Nangong, Xiu-Jun Lu, Xiu-Hua Li, Guo-Xun Li and Long Cui (2005)
[Purification of insecticidal proteins from Xenorhabdus nematophila HB310 and detection of their insecticidal activity]
Acta Entomologica Sinica 48 (3), 353-358
Abstract: The extracts of extracellular and intracellular insecticidal proteins from Xenorhabdus nematophila HB310 strain, an entomopathogenic bacterium symbiotically associated with entomopathogenic nematode Steinernema carpocapsae, appeared high toxic activity by oral or injectable infection against several species of insects. Two oral insecticidal proteins, toxinIand toxinII, and another haemocoel insecticidal protein, toxin III with injectable activity were purified from the intracellular protein extract by native-PAGE. The same three protein toxins also were obtained from the extracellular protein extract. There was only a single band for any of toxins above, but the results of SDS-PAGE showed four bands for toxin I, three bands for toxin II and one band for toxin III, respectively. Bioassay results indicated that the oral insecticidal activity of toxin II was higher than that of toxin I to Helicoverpa armigera neonates. The injectable hemocoelic potency (LD₅₀) of toxin III was 0.18 µg/larva against Galleria mellonella fifth-instar larvae. The toxicity of toxin II was stable under 50°C in 10 min. The toxin III was heat-stable to 70°C in 10 min.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): QinYing Wang

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera
Steinernema carpocapsae/Xenorhabdus nematophila (entomopathogen)	Helicoverpa armigera