Acta Entomologica Sinica (2005) 48, 347-352

Jia-Ping Xu, Ke-Ping Chen, Qin Yao, Qing-Gang Xu, Xiao-Yong Liu and Gui-Tian Gao (2005)
[Fluorescent differential display analysis of the gene s3a related to NPV resistance in Bombyx mori L.]
Acta Entomologica Sinica 48 (3), 347-352
Abstract: Using fluorescent differential display (FDD) technique, the differential expression of genes related to BmNPV resistance in highly resistant strain NB, highly susceptible strain 306 and near isogenic line 306NNZZ of the silkworm, Bombyx mori L. was analyzed. Based on the differential display bands, a 702 bp fragment named C18₇₀₂ was cloned and confirmed by Northern blot hybridizations. The sequence was then electrically extended based on homology comparison with NCBI ESTs, and further confirmed by RT-PCR using specific primers. A novel gene was characterized and revealed to encode a putative BmS3A protein. The gene had 97.7% homology to Spodoptera frugiperda S3A, 94% homology to Heliothis virescens S3A and 75.3% homology to Drosophila melanogaster S3A, and was named B. mori s3a(Bms3a). It had higher expression in the highly resistant strain and near isogenic line than in the highly susceptible silkworm strain, and it also had higher expression in BmNPV treated strains than untreated strains. The results suggested that Bms3a is involved in silkworm BmNPV resistance.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Alphabaculovirus bomori