Acta Entomologica Sinica (2005) 48, 342-346

Ji-Ning Liu, Xian-Jin Liu, Xiang-Yang Yu and Zheng-Qiang Peng (2005)
[Construction of recombined plasmid carrying Cry1Ac gene and expression of insecticidal activities in Bacillus subtilis]
Acta Entomologica Sinica 48 (3), 342-346
Abstract: The full length sequence of the promoter and Cry1Ac gene were obtained by PCR with two pairs of unique primers Cry1Ac F/R and Pxy F/R respectively, which were designed according to the Cry1Ac gene and promoter sequence of xylase operon from Bacillus subtilis 168 Then, the fused translational expression vector PxylR-Cry1Ac was constructed using overlapping PCR technique with the primers pair PxyF/Cry1AcR and the mixture of above PCR production. After being digested by SphI and BamHI, PxylR-Cry1Ac expression vector was inserted into E. coli-B. thuringiensis shuttle vector pHT315, and the resulted recombinant plasmids were named as pCry1Ac315 The recombinant plasmids were transferred into B. subtilis laboratory strain JAAS01D. Efficient expression of the Cry1Ac gene in the engineered JAAS01D-1Ac was proved with restriction enzyme analysis, SDS-PAGE electrophoresis analysis and insecticidal activity assay.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zheng-Qiang Peng

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
genetical engin./transgenic plants
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Bacillus thuringiensis Cry1A-toxin (entomopathogen)