Acta Entomologica Sinica (2005) 48, 13-17

Bo Gao, Zhi-Gang Liu, Miao Xing, Hong Xu, Shi-Wen Luo and Ren Lai (2005)
[Expression, purification and characterization of the American cockroach Cr PI allergen]
Acta Entomologica Sinica 48 (1), 13-17
Abstract: Using the Cr PI clone from the lambdaEXcell library as a template, the cDNA fragments were first generated by PCR techniques and then ligated into T vector. After being confirmed by DNA sequencing, the cDNA encoding the American cockroach Cr PI allergen was subcloned into pGEX-5X-1 and expressed as GST-fusion protein in the form of inclusion bodies. After being dissolved in 6 mol/L guanidine hydrochloride and renatured with a simple dilution method, the proteins of target were purified to above 90% purity by affinity chromatography with Glutathione Sepharose 4B. Tested with sera from subjects allergic to cockroach, the recombinant allergen was shown to possess good IgE-binding activity as determined by Western blotting.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Periplaneta americana