Acta Entomologica Sinica (2004) 47, 639-644
Jin-Hu Geng, Zheng-Xi Li and Zuo-Rui Shen (2004)
[Application of species-specific primers to molecular identification of three important Trichogramma species (Hymenoptera : Trichogrammatidae) in China]
Acta Entomologica Sinica 47 (5), 639-644
Abstract: Species-specific primers of Trichogramma chilonis Ishii has been designed based on its rDNA-ITS2 sequence in this paper, and the specific primers of T. dendrolimi Matsumura, T. ostriniae Pang et Chen and the genus Trichogramma were retrieved from previously published articles. All of the specific primers were then applied to identification of multiple Trichogramma and host eggs samples through PCR assays. The results showed that our methods could produce clear, unique and reproducible target DNA bands from individual adult wasp. Furthermore, the species-specific primer-based results were fully consistent with those derived from morphology-based ones. Thus, the molecular identification of the three important Trichogramma species in China, namely T. dendrolimi, T. chilonis, and T. ostriniae, using the above-described diagnostic primers proved feasible.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zuo Rui Shen, Zheng-Xi Li
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
identification/taxonomy
surveys/distribution/isolation
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Trichogramma chilonis (parasitoid) | ||||
Trichogramma ostriniae (parasitoid) | ||||
Trichogramma dendrolimi (parasitoid) |