Acta Entomologica Sinica (2003) 46, 150-155

Jiang Yao, Jie Zhang, Zhong-Yi Chen, Fu-Ping Song, Chang-You Li, Yu-Qin Hu and Da-Fang Huang (2003)
[Cloning and expression of a Bt cry1Aa gene with high toxicity against lepidopterous pests]
Acta Entomologica Sinica 46 (2), 150-155
Abstract: Bacillus thuringiensis strain Ly30, isolated from Lianyungang, Jiangsu Province, was highly toxic to several kinds of insect pests. Results of CAPS (cleaved amplified polymorphic sequences) analysis indicated that Ly30 contained the cry1Aa gene. According to the 5' and 3' end nucletiode sequence of the known cry1Aa gene, one pair of primers was designed and the full-length cry1Aa gene (3.5 kb) was thereby obtained by PCR amplification from strain Ly30. Sequencing analysis showed that this cry 1Aa gene contained one open reading frame of 3 531 bp, and encoded 1 176 amino acid residues deduced from its nucletiode sequence with molecular mass of 133.2 kD. This gene was registered in Gen-Bank (Accession number is AF384211) and designated as cry1Aa12 , a novel cry gene, by the International Nomenclature Committee of Bt delta-endotoxin genes. Expression of cry1Aa12 had been performed by inserted it into E. coli expression vector pKK233-2 , and a 133.2 kD protein band could be easily detected by SDS-PAGE. Results of bioassays proved that the expression products of cry1Aa12 gene had high toxicity against two Plutella xylostella colonies, with LC50 of 0:203 µg/mL, 0.554 µg/mL respectively.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Jiang Yao, Jie Zhang

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
genetical engin./transgenic plants

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Plutella xylostella
Bacillus thuringiensis Cry1A-toxin (entomopathogen)	Plutella xylostella		China (NE)