Acta Entomologica Sinica (2002) 45, 285-289

Gui-Rong Wang, Yu-Yuan Guo and Kong-Ming Wu (2002)
Expression and identification of general odorant binding protein II from Helicoverpa armigera (Hübner)
Acta Entomologica Sinica 45 (3), 285-289
Abstract: The intact open-reading fragment sequence of mature GOBP2-Harm from Helicoverpa armigera was obtained by PCR amplification. The ORF of GOBP2-Harm was subcloned into expression vector pGEX-4T-1. Recombinant protein was expressed successfully in E. coli induced by IPTG. The analysis of SDS-PAGE showed that most of recombinant protein was insoluble inclusion body and only little fraction of recombinant protein of GOBP2-Harm is soluble. In order to obtain abundant soluble recombinant protein, the insoluble inclusion body GOBP2-Harm was solubilized, refolded and purified. The purified product was crossreactive with an anti-GOBP (Antheraea polyphemus) antiserum, which indicated the purified protein belonged to GOBP of insect.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Kong Ming Wu, Yu Yuan Guo

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Helicoverpa armigera