Acta Entomologica Sinica (1998) 41, 337-342

Zhiming Yuan, C. Nielsen-LeRoux, N. Pasteur, J.F. Charles and R. Frutos (1998)
[Detection of the binary toxin genes of several Bacillus sphaericus strains and their toxicities against susceptible and resistant Culex pipiens]
Acta Entomologica Sinica 41 (4), 337-342
Abstract: Using synthetic oligonucleotides designed on the basis of the binary toxin gene sequence of 2362, a 1.1 kilodalton DNA fragment has been prepared as a probe to study the toxin genes of several Bacillus sphaericus strains. The 3.5kb Hind III DNA fragments of C₃-41, IAB881, IAB872 and BS-197 as well as the 4. 7kb Hind III DNA fragment of LP1-G are homologous to the probe. The 51.4 and 41.9 kilodalton binary toxin can be detected by SDS-PAGE and Western Blotting. The results of the bioassay showed that C₃-41, IAB881, BS-197 and the reference strain 2362 have a high potency against susceptible Culex pipiens subsp. pipiens and no potency against the resistant mosquito larvae from France (SPHRAE). IAB872 and LP1-G have medium toxicity against the resistant larvae.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: Chinese)
Database assignments for author(s): Zhi Ming Yuan, Christina Nielsen-Leroux

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance/tolerance/defence of host
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Culex pipiens
Lysinibacillus sphaericus (entomopathogen)	Culex pipiens