Journal of Phytopathology (2018) 166, 403-411
Andre Freire Cruz, Geleta Dugassa Barka, Justine Sylla and Annette Reineke (2018)
Biocontrol of strawberry fruit infected by Botrytis cinerea: Effects on the microbial communities on fruit assessed by next-generation sequencing
Journal of Phytopathology 166 (6), 403-411
Abstract: Fruit grey mould, caused by the fungus Botrytis cinerea, is known to be a harmful disease of strawberry at postharvest stage. However, effects of an application of biological control agents (BCAs) on strawberry fruit in terms of shift in the microbial community are still unknown. The present research aimed to investigate the effects of an application of BCAs on postharvest microbial populations present on strawberry fruits. Strawberry plants were sprayed with three kinds of BCA, RhizoVital 42 fl. (Bacillus amyloliquefaciens FZB42), Trianum-P (Trichoderma harzianum T22) and Naturalis (Beauveria bassiana ATCC 74040), targeting Botrytis cinerea fungus. Control plots were composed of water and fungicide treatments. Microbial communities (bacteria and fungi) were analysed via next-generation sequencing on an Illumina MiSeq. Analysis of 16S RNA and ITS rRNA sequences indicated that the BCAs application modified both bacterial and fungal community compositions and diversity. An application of two BCAs together had more effects on microbial community composition than a single application. These results suggest that BCAs can modify bacterial and fungal community composition and diversity on strawberry fruits, which may consequently improve the efficiency and establishment of these products on control of postharvest diseases of fruits, such as grey mould.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
(original language: English)
Link to article at publishers website
Database assignments for author(s): Annette Reineke
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
environment/habitat manipulation
non-target effects/fate in environm.
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Botrytis cinerea | Strawberry (Fragaria) | |||
| Trichoderma harzianum (antagonist) | Botrytis cinerea | Strawberry (Fragaria) | ||
| Bacillus amyloliquefaciens (antagonist) | Botrytis cinerea | Strawberry (Fragaria) | ||
| Beauveria bassiana (antagonist) | Botrytis cinerea | Strawberry (Fragaria) |
