Annals of Applied Biology (2002) 140, 21-28

C.I. Dovas, A.P. Mamolos and N.I. Katis (2002)
Fluctuations in concentration of two potyviruses in garlic during the growing period and sampling conditions for reliable detection by ELISA
Annals of Applied Biology 140 (1), 21-28
Abstract: To optimise sampling conditions for the detection by ELISA of Onion yellow dwarf virus (OYDV) and Leek yellow stripe virus (LYSV), the most important viral pathogens of garlic worldwide, relative virus concentrations were determined during the growing period and in different leaf parts by DASELISA. Both viruses were found to have uneven distributions in garlic plants, with the tips of the two latest fully developed leaves showing the highest concentrations and the oldest leaves the lowest concentrations. The tips of the youngest leaves were found to have higher virus concentrations than their middle and basal sections. In the older leaves, viruses were distributed more uniformly in the three leaf sections. In the oldest leaves virus levels in the leaf tips were significantly decreased. The concentrations of OYDV and LYSV increased until March, whereas later on they decreased. During storage of leaf samples at 6°C for 15 days, a loss was found of both virus antigens of more than 80% , and during 109 days of storage at -30°C a loss of more than 90% was found.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Crisostomos I. Dovas

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Onion yellow dwarf virus	Onion/garlic/leek (Allium)
	Leek yellow stripe virus	Onion/garlic/leek (Allium)