Annals of Applied Biology (2020) 176, 203-209

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Romana Iftikhar, Lindani Moyo and Hanu R. Pappu (2020)
A loop-mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in dahlia (Dahlia variabilis)
Annals of Applied Biology 176 (2), 203-209
Abstract: Dahlia mosaic virus (DMV), an aphid-transmitted caulimovirus in the family Caulimoviridae, is an important pathogen of dahlia (Dahlia variabilis). Development of rapid, and sensitive diagnostic tools is essential in surveillance and management of DMV. A loop-mediated isothermal amplification (LAMP) assay was developed for DMV detection. The LAMP assay targets the reverse transcriptase region of the DMV genome and is optimal at a temperature of 60°C and run time of 60 min, and amplification was detected through fluorescence detection and by agarose gel electrophoresis. The assay detected DMV in DNA concentration as low as 10-3 ng. To the best of our knowledge, this is the first report of a LAMP assay for the detection of DMV, a plant DNA virus. This assay will be useful in rapid and sensitive detection of DMV and in reducing the virus incidence through production of virus-free planting material.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Hanu R. Pappu

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Caulimovirus tessellodahliae Dahlia (crop)
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