Annals of Applied Biology (2020) 176, 203-209

Romana Iftikhar, Lindani Moyo and Hanu R. Pappu (2020)
A loop-mediated isothermal amplification assay for the detection of Dahlia mosaic caulimovirus in dahlia (Dahlia variabilis)
Annals of Applied Biology 176 (2), 203-209
Abstract: Dahlia mosaic virus (DMV), an aphid-transmitted caulimovirus in the family Caulimoviridae, is an important pathogen of dahlia (Dahlia variabilis). Development of rapid, and sensitive diagnostic tools is essential in surveillance and management of DMV. A loop-mediated isothermal amplification (LAMP) assay was developed for DMV detection. The LAMP assay targets the reverse transcriptase region of the DMV genome and is optimal at a temperature of 60°C and run time of 60 min, and amplification was detected through fluorescence detection and by agarose gel electrophoresis. The assay detected DMV in DNA concentration as low as 10^-3 ng. To the best of our knowledge, this is the first report of a LAMP assay for the detection of DMV, a plant DNA virus. This assay will be useful in rapid and sensitive detection of DMV and in reducing the virus incidence through production of virus-free planting material.
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Link to article at publishers website
Database assignments for author(s): Hanu R. Pappu

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Caulimovirus tessellodahliae	Dahlia (crop)