Journal of Pest Science (2013) 86, 151-155

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Shun-Ichiro Takano, Atsushi Mochizuki, Keiji Takasu, Kazuhiko Konishi, Jelfina C. Alouw, Donata S. Pandin and Satoshi Nakamura (2013)
Rapid discrimination of two cryptic species within Brontispa longissima (Gestro) (Coleoptera: Chrysomelidae) by PCR-RFLP
Journal of Pest Science 86 (2), 151-155
Abstract: Brontispa longissima (Coleoptera: Chrysomelidae) is a serious invasive pest of coconut palm (Cocos nucifera) supposedly originating from Indonesia and New Guinea. It has recently invaded Southeast and East Asia, where it has caused serious damage to coconut plants. Brontispa longissima as currently defined contains two cryptic species: we herein referred to one as the "Asian clade", which is distributed over a wide area, including Asia and the Pacific region; and we referred to the other one as the "Pacific clade", which is found in a limited area in the Pacific region. We developed a PCR-RFLP method for differentiating the two clades. Digestion of the PCR product of a 1,014-bp region within the mitochondrial cytochrome oxidase I gene (COI) with BslI, HpyCH4III, or NlaIV resulted in clade-specific patterns as estimated by the sequence data. We applied the method to specimens newly obtained from various locations to investigate the geographical distribution of B. longissima. Although B. longissima collected from Samoa in April 2003 had been placed in the Pacific clade, specimens collected from the same island in April 2010 were placed in the Asian clade, suggesting that the predominant clade may have been changing from the former to the latter. On Timor, specimens included both clades in apparently segregated habitats.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Satoshi Nakamura, Keiji Takasu, Atsushi Mochizuki, Kazuhiko Konishi

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Brontispa longissima Coconut (Cocos nucifera) Samoa
Brontispa longissima Coconut (Cocos nucifera) East Timor
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